THE E6 AND E7 GENES OF HPV-18 ARE SUFFICIENT FOR INDUCING 2-STAGE INVITRO TRANSFORMATION OF HUMAN KERATINOCYTES
- 1 December 1989
- journal article
- research article
- Vol. 4 (12) , 1529-1532
Abstract
Using a recently described keratinocyte assay, we demonstrate that HPV-18 DNA induces two progressive steps in cellular transformation (a large cell and a small cell stage). Both steps of this keratinocyte transformation can be achieved with a subgenomic fragment containing only the HPV-18 regulatory region and E6/E7 genes. Similar to cell lines transformed by the complete HPV-18 genome, keratinocytes transformed by the HPV-18 E6/E7 genes express the major early viral protein (E7) but are non-tumorigenic in nude mice. Interestingly, HPV-18 DNA was noted to be 5 times more efficient than HPV-16 DNA for in vitro keratinocyte transformation, regardless of the method of DNA transfection.This publication has 22 references indexed in Scilit:
- Characterization of primary human keratinocytes transformed by human papillomavirus type 18Journal of Virology, 1988
- The human papillomavirus type 16 E7 gene encodes transactivation and transformation functions similar to those of adenovirus E1ACell, 1988
- Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.Proceedings of the National Academy of Sciences, 1987
- The E6-E7 region of human papillomavirus type 18 is sufficient for transformation of NIH 3T3 and rat-1 cellsJournal of Virology, 1987
- Transcription of the cottontail rabbit papillomavirus early region and identification of two E6 polypeptides in COS-7 cellsJournal of Virology, 1987
- Identification of the HPV-16 E6 protein from transformed mouse cells and human cervical carcinoma cell lines.The EMBO Journal, 1987
- Transformation of human fibroblasts and keratinocytes with human papillomavirus type 16 DNAJournal of Virology, 1987
- MOLECULAR AND CYTOGENETIC ANALYSIS OF IMMORTALIZED HUMAN PRIMARY KERATINOCYTES OBTAINED AFTER TRANSFECTION WITH HUMAN PAPILLOMAVIRUS TYPE-16 DNA1987
- Transcription of human papillomavirus type 16 early genes in a cervical cancer and a cancer-derived cell line and identification of the E7 protein.Proceedings of the National Academy of Sciences, 1986
- Enhancer-dependent expression of human kappa immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation.Proceedings of the National Academy of Sciences, 1984