Proteolytic enzyme sensors using an ion-sensitive field effect transistor.
- 1 January 1988
- journal article
- research article
- Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN
- Vol. 36 (3) , 1190-1193
- https://doi.org/10.1248/cpb.36.1190
Abstract
Two kinds of enzyme sensors were fabricated by chemically immobilizing an enzyme on the gate surface of an ion-sensitive field effect transistor (ISFET). One is a trypsin-membrane/ISFET sensor which can measure concentrations of N-benzoyl-L-arginine ethyl ester in the range from 5 .times. 10-4 to 5 .times. 10-2 M. The other is an .alpha.-chymotrypsin-membrane/ISFET sensor, which can measure concentrations of N-acetyl-L-tyrosine ethyl ester solution in the range of 5 .times. 10-4.sbd. 5 .times. 10-3 M. The response of the sensors was affected markedly by pH and the concentration of the working buffer.This publication has 2 references indexed in Scilit:
- Urea sensor based on an ion-sensitive field effect transistor. IV Determination of urea in human blood.CHEMICAL & PHARMACEUTICAL BULLETIN, 1987
- Field effect transistor sensitive to penicillinAnalytical Chemistry, 1980