Comparison of Serum Fructosamine with Glycosylated Serum Protein (Determined by Affinity Chromatography) for the Assessment of Diabetic Control

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Abstract
Glycosylated total protein (GTP) and glycosylated albumin (GALb) were measured in serum using aminophenylboronic acid affinity chromatography and the results were compared with those found using the fructosamine assay. The percentage GTP and GALb found by affinity chromatography correlated well with fructosamine values in the sera of a group of non-diabetic and diabetic patients (fructosamine vs GTP, r = 0.91, p<0.001; fructosamine vs GALb, r = 0.91, p<0.001). Results of each method gave similar correlations when compared with the degree of diabetic control assessed by glycosylated haemoglobin (GHb) and fasting plasma glucose (FPG) (fructosamine vs FPG, r = 0.74, p<0.001; GTP vs FPG, r = 0.75, p<0.001; GALb vs FPG, r = 0.79, p<0.001; fructosamine vs GHb, r = 0.79, p<0.001; GTP vs GHb, r = 0.81, p<0.001; GALb vs GHb, r = 0.84, p<0.001). Both methods could equally discriminate between groups of non-diabetics and diabetic patients (p<0.001) and showed similar temporal changes after starting insulin therapy.