Rapid In Vivo Assay for Friend Polycythemia Virus 23
- 1 April 1974
- journal article
- research article
- Published by Oxford University Press (OUP) in JNCI Journal of the National Cancer Institute
- Vol. 52 (4) , 1223-1229
- https://doi.org/10.1093/jnci/52.4.1223
Abstract
An assay for the Friend polycythemia virus (FVP) was developed based on the capacity of plasma from FVP-infected animals to increase greatly the splenic uptake of 59Fe as early as 3 days after infection in fasted BALB/c mice. Our experiments supported the following conclusions: 1) The increase in splenic 59Fe uptake represented a proportional increase in hemoglobin synthesis. 2) The 59Fe response was not due to the presence of erythropoietin in the FVP plasma or to the induction by FVP of endogenous erythropoietin production. 3) The 59Fe assay correlated well with the spleen focus-forming assay. 4) The assay had applicability in monitoring FVP purification procedures and studying physicochemical properties of FVP.Keywords
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