Accelerated Immunogold Silver and Immunoperoxidase Staining of Paraffin Sections with the Use of Microwave Irradiation: Factors Influencing Results
Open Access
- 1 February 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in American Journal of Clinical Pathology
- Vol. 91 (2) , 137-143
- https://doi.org/10.1093/ajcp/91.2.137
Abstract
With the use of microwave irradiation, incubation times for the immunotechniques used in immunomicroscopy can be shortened significantly. The authors found that factors influencing results are irradiation time, power output, and presence of gold label. Precise temperature control is essential, and techniques to achieve this are discussed. For beta–human chorionic gonadotropin (β-HCG), the results with the immunoperoxidase technique were inferior to those of the conventional technique, and with the immunogold silver technique an enhancement of labeling results was achieved. Immunogold silver staining for β-HCG on paraffin sections can be performed with the use of microwave irradiation within one hour.Keywords
This publication has 1 reference indexed in Scilit: