Leucyl Transfer RNA Synthetase Changes during Soybean Cotyledon Senescence
- 1 January 1971
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 68 (1) , 224-228
- https://doi.org/10.1073/pnas.68.1.224
Abstract
Previous work has shown the complement of leucyl transfer RNA (tRNA Leu ) isoaccepting species present in soybean cotyledons to change during cotyledon senescence. Of the six tRNA Leu species resolved by Freon-column chromatography, the capacity of species 1-4 to accept leucine is decreased between 5 and 21 days of age. Parallel changes in tRNA Leu synthetase activity are reported here during the same time period. There is a reduction in the capacity of the synthetase from 21-day-old cotyledons to aminoacylate tRNA Leu 1-4, while the capacity to charge tRNA Leu 5-6 remains high. When tRNA Leu derived from 5-day-old cotyledons is maximally charged with the 21-day synthetase, the subsequent addition of synthetase from 5-day-old cotyledons promotes additional charging, primarily of species 1-4. These results suggest the presence of tRNA Leu “subspecies” within the individual peaks resolved on a Freon column, differing in synthetase specificity.Keywords
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