Tracing functionally identified neurones in a multisynaptic pathway in the hamster and rat using herpes simplex virus expressing green fluorescent protein
Open Access
- 1 November 2001
- journal article
- Published by Wiley in Experimental Physiology
- Vol. 86 (6) , 695-702
- https://doi.org/10.1111/j.1469-445x.2001.tb00034.x
Abstract
Using a genetically modified herpes simplex virus encoding green fluorescent protein we sought to establish if this viral modification could be used in transneuronal tracing studies of the sympathetic nervous system. The herpes simplex virus encoding green fluorescent protein was injected into the adrenal medulla of three hamsters and six rats. After a suitable survival period, neurones in the sympathetic intermediolateral cell column of the thoracolumbar spinal cord, rostral ventral medulla and paraventricular nucleus of the hypothalamus were clearly identified by the presence of a green fluorescence in the cytoplasm of the neurones of both species. Thus, herpes simplex virus encoding green fluorescent protein labelled chains of sympathetic neurones in the hamster and rat and therefore has the potential to be used in transneuronal tracing studies of autonomic pathways in these species.Keywords
This publication has 45 references indexed in Scilit:
- Arrangement of dendrites and morphological characteristics of sympathetic preganglionic neurones projecting to the superior cervical ganglion and adrenal medulla in adult catJournal of the Autonomic Nervous System, 1995
- Spinal cord lamina V and lamina VII interneuronal projections to sympathetic preganglionic neuronsJournal of Comparative Neurology, 1994
- A comparison between the adult rat and neonate rat of the architecture of sympathetic preganglionic neurones projecting to the superior cervical ganglion, stellate ganglion and adrenal medullaJournal of the Autonomic Nervous System, 1994
- Specificity of pseudorabies virus as a retrograde marker of sympathetic preganglionic neurons: implications for transneuronal labeling studiesBrain Research, 1993
- Peripheral and central pathways regulating the kidney: a study using pseudorabies virusBrain Research, 1993
- Transneuronal transfer of herpes simplex virus type 1 (HSV 1) from mixed limb nerves to the CNS. I. Sequence of transfer from sensory, motor, and sympathetic nerve fibres to the spinal cordJournal of Comparative Neurology, 1992
- Preganglionic sympathetic neurones innervating the rat adrenal medulla: immunocytochemical evidence of synaptic input from nerve terminals containing substance P, GABA or 5-hydroxytryptamineJournal of the Autonomic Nervous System, 1988
- The paraventricular nucleus of the hypothalamus: Cytoarchitectonic subdivisions and organization of projections to the pituitary, dorsal vagal complex, and spinal cord as demonstrated by retrograde fluorescence double‐labeling methodsJournal of Comparative Neurology, 1980
- Evidence for Herpes Simplex Virus Type-selective Receptors on Cellular Plasma MembranesJournal of General Virology, 1979
- Direct hypothalamo-autonomic connectionsBrain Research, 1976