Fibronectin remains in the cytoskeletal preparations of cultured human fibroblasts

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Abstract
Cytoskeleton models were produced by treating cultured human embryonal fibroblasts with Triton X-100 and actomyosin dissociating buffers. Morphological studies indicated that in addition to nuclear residues and intermediate filaments, both plasma membrane remnants and substratum-associated, pericellular material remained on the culture substratum. By immunochemical analysis the extracellular material was identified as fibronectin. The results suggest that fibronectin is associated with the nucleus-anchoring cytoskeleton of cultured human fibroblasts.

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