Abstract
A new enzymatic method for direct photometric determination of oxalate in serum and urine is described, using oxalate oxidase. The resulting H2O2 is measured with a coupled enzyme system of catalase [EC 1.11.1.6] and aldehyde dehydrogenase [EC 1.2.1.5]. Percentage recovery of added oxalate was 99 .+-. 4 in serum, and 98 .+-. 4 in urine (n = 10). Serum oxalate levels varied from 16.9 to 44.8 .mu.mol/l. Oxalate values can be determined within 20 min, without time consuming pretreatment of samples. The detection limit is 5 .mu.mol/l.

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