3‐Methyladenine, an inhibitor of autophagy, has multiple effects on metabolism
- 1 August 1988
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 175 (2) , 325-329
- https://doi.org/10.1111/j.1432-1033.1988.tb14200.x
Abstract
3-Methyladenine is generally used as an inhibitor of autophagy [P. O. Seglen and P. B. Gordon (1982) Proc. Natl Acad. Sci. USA 79, 1889-1892]. Using isolated hepatocytes, we observed that 3-methyladenine has other effects as well. 1. 3-Methyladenine promoted glycogen breakdown and inhibited flux through phosphofructokinase and pyruvate kinase. These effects proved to be unrelated to inhibition of autophagic proteolysis and were caused by cAMP, which slightly increased in the presence of 3-methyladenine. 2. Addition of 3-methyladenine to intact hepatocytes increased the intralysosomal pH and caused a lower density of the lysosomal population upon centrifugation in a Percoll density gradient. No increase in the intralysosomal pH was effected by 3-methyladenine in isolated lysosomes.This publication has 27 references indexed in Scilit:
- Energy dependence of autophagic protein degradation in isolated rat hepatocytesEuropean Journal of Biochemistry, 1987
- Amino acid control of autophagic sequestration and protein degradation in isolated rat hepatocytes.The Journal of cell biology, 1984
- GLUCONEOGENESIS AND RELATED ASPECTS OF GLYCOLYSISAnnual Review of Biochemistry, 1983
- Intracellular Compartment ation and Control of Alanine Metabolism in Rat Liver Parenchymal CellsEuropean Journal of Biochemistry, 1982
- Amino acid inhibition of the autophagic/lysosomal pathway of protein degradation in isolated rat hepatocytesBiochimica et Biophysica Acta (BBA) - General Subjects, 1980
- The Relationship between Intramitochondrial N‐Acetylglutamate and Activity of Carbamoyl‐Phosphate Synthetase (Ammonia)European Journal of Biochemistry, 1980
- Effect of 2-deoxyglucose on lysosomal enzymes in cultured human skin fibroblastsExperimental Cell Research, 1980
- The permeability properties of the lysosomal membraneBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1977
- Rate-Limiting Steps of Gluconeogenesis in Liver Cells as Determined with the Aid of Fluoro-Dicarboxylic AcidsEuropean Journal of Biochemistry, 1972
- HIGH-YIELD PREPARATION OF ISOLATED RAT LIVER PARENCHYMAL CELLSThe Journal of cell biology, 1969