Indigenous Plasmids inXanthomonas campestrispv.citri

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Abstract
Indigenous plasmid DNA was isolated from X. campestris pv. citri by lysis of cells in alkaline-SDS [sodium dodecyl sulfate]. The plasmids were detected by electrophoresis in horizontal agarose slab gels. At least 7 plasmids with apparent molecular masses of approximately 12-53 megadaltons occurred in 6 laboratory reference strains and 21 clinical strains of diverse host, geographical, and temporal origin. Plasmids with the same or similar electrophoresis mobility in the 6 reference strains were distributed in 5 unique plasmid profiles that were associated with different forms of citrus bacterial canker disease (CBCD). However, no specific phenotypic function(s) was associated with any of these plasmids of X. c. pv. citri. Plasmid profiles may be useful in epidemiological studies of this pathogen, supplementing other methods of bacterial detection and identification. Furthermore, these plasmids may be useful for evaluating relationships between strains of X. c. pv. citri associated with different forms of CBCD.