Generation of hepatocytes expressing functional cytochromes P450 from a pancreatic progenitor cell line in vitro
- 15 March 2003
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 370 (3) , 763-769
- https://doi.org/10.1042/bj20021545
Abstract
The proliferating AR42J-B13 pancreatic cell line is known to respond to glucocorticoid treatment by producing foci of cells that express the liver-specific albumin gene. We demonstrate that this cell line also expresses liver-specific or liver-enriched functional cytochrome P450 proteins when stimulated to trans-differentiate into hepatocytes by glucocorticoid. These data suggest that this cell line has an unusual ability to trans-differentiate into functional hepatocytes and that it could be possible to generate a limitless supply of functional hepatocyte-like cells in vitro.Keywords
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