Close Linkage of the Genes serC (for Phosphohydroxy Pyruvate Transaminase) and serS (for Seryl-Transfer Ribonucleic Acid Synthetase) in Escherichia coli K-12
- 1 March 1973
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 113 (3) , 1091-5
- https://doi.org/10.1128/jb.113.3.1091-1095.1973
Abstract
Escherichia coli strain K28, isolated after nitrosoguanidine mutagenesis, was found to be auxotrophic for serine. It was also temperature sensitive for growth as a result of producing an altered seryl-transfer ribonucleic acid (tRNA) synthetase (EC 6.1.1.11, l-serine: tRNA ligase [AMP]). The auxotrophy was caused by a mutation in the structural gene for phosphohydroxy-pyruvate transaminase (serC), which was distinct from, but closely linked to, the structural gene for seryl-tRNA synthetase (serS). We conclude that the relevant genes are in the order gal-serS-serC-aroA.Keywords
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