MEIOTIC DIPLOID PROGENY AND MEIOTIC NONDISJUNCTION IN SACCHAROMYCES CEREVISIAE
Open Access
- 30 April 1982
- journal article
- research article
- Published by Oxford University Press (OUP) in Genetics
- Vol. 101 (1) , 17-33
- https://doi.org/10.1093/genetics/101.1.17
Abstract
Abnormalities in chromosome number that occurred during meiosis were evaluated with a specially-constructed diploid strain of Saccharomyces cerevisiae. The strain is heterozygous for six markers of the right arm of chromosome V and heterozygous for cyh2 (resistance to cycloheximide) on chromosome VII.—Selection of meiotic spores on a medium containing cycloheximide and required nutrilites—except those for the markers of the right arm of chromosome V—allows the growth of aberrant clones belonging only to two classes: a) diploid clones, caused by failure of the second meiotic division, with a frequency of 0.54 × 10-4 per viable spore; and b) diplo V, aneuploids derived from nondisjunctions in meiosis I or meiosis II, with a total spontaneous frequency of 0.95 × 10-4 per viable spore. About two-thirds of the aneuploids originated during meiosis I, the rest during meiosis II. An investigation of these events in control meioses and after treatment with MMS, Benomyl and Amphotericin B suggests that this assay system is suitable for screening environmental mutagens for their effects on meiotic segregation.This publication has 9 references indexed in Scilit:
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