REVERSAL OF TOXIC AND ANOXIC INDUCED HEPATIC-FAILURE BY SYNGENEIC, ALLOGENEIC, AND XENOGENEIC HEPATOCYTE TRANSPLANTATION

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  • 1 January 1980
    • journal article
    • research article
    • Vol. 88  (2) , 244-253
Abstract
The efficacy of syngeneic hepatocyte transplantation in an anoxic model and of xenogeneic (rabbit and porcine) hepatocyte transplnatation in a toxic model of fulminant hepatic failure in the rat is described. Lewis strain rats that received 4 .times. 107 hepatocytes i.p. at 48 h after hepatic artery ligation had a significantly improved survival rate (79%, no. = 14) when compared with control animals (38%, no. = 21, P < 0.05). Xenogeneic hepatocytes (4 .times. 107 cells/rat) given to D-galactosamine-poisoned Lewis rats at 48 h after toxin administration were able to significantly improve survival rate as compared with controls (71% vs. 14%, no. = 14 P < 0.01 for rabbit; and 75%, no. = 14 vs. 12.5%, no. = 16, P < 0.001 for porcine). Although an increase in in vivo cytotoxicity could be demonstrated after porcine hepatocyte transplantation, no adverse clinical effects were observed. The methodology for the harvest and storage of large numbers of hepatocytes from a large animal liver was developed and it is now feasible to proceed to the clinical application of hepatocyte transplantation for human fulminant hepatic failure.