High-Speed DNA Sequencing: An Approach Based Upon Fluorescence Detection of Single Molecules
- 1 October 1989
- journal article
- research article
- Published by Taylor & Francis in Journal of Biomolecular Structure and Dynamics
- Vol. 7 (2) , 301-309
- https://doi.org/10.1080/07391102.1989.10507773
Abstract
We are developing a laser based technique for the rapid sequencing of large fragments (∼40 kb) of DNA based upon the detection of single, fluorescently tagged nucleotides cleaved from a single DNA fragment. We have demonstrated significant progress on several of the important steps of this technique. The projected rate of sequencing is several hundred bases per second which is orders of magnitude faster than existing methods. Once developed, this technology could be utilized by investigators for rapid sequencing of genetic material from virtually any source.This publication has 20 references indexed in Scilit:
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