Differences in the Production of Arachidonic Acid Metabolites between Healthy and Rheumatic Synovial FibroblastsIn Vitro:A Preliminary Study

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Abstract
Production of various arachidonic acid metabolites from both endogenous and exogenous substrate was measured using cultures of synovial fibroblasts from healthy and rheumatic synovia. At first, the rheumatic cells showed retarded growth and an altered histological picture. Rheumatic cells produced more 6-keto-PGF1a, the main metabolite of prostacyclin, and prostaglandin E2 than did normal cells, which synthesized more thromboxane B2. Later on these differences diminished or disappeared, except regarding 6-keto-PGF1a. When fairly high concentrations of exogenous arachidonic acid were used, for 2-hour incubation of the cells, the production of identified metabolites, 6-keto-PGF1a, PGF2a, PGE2, PGD2, PGA+PGB and thromboxane B2, was slightly less in rheumatic cells. In general, the main metabolite formed was 6-keto-PGF1a. Some kind of feedback mechanism between prostaglandins and cyclic nucleotides is suggested.