Luminol chemiluminescence and peroxidation of unsaturated fatty acid induced by the xanthine oxidase system: Effect of oxygen radical scavengers.

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Abstract
Addition of luminol to the xanthine oxidase system caused the production of chemiluminescence (CL). Superoxide dismutase (SOD) and singlet oxygen (1O2) scavengers inhibited the CL, whereas catalase and hydroxyl radical (HO.cntdot.) scavengers had no significant effect, suggesting that 1O2 species derived from O2- is responsible for the light emission of luminol. Addition of unsaturated acids to the xanthine oxidase system in the presence of luminol resulted in quenching of CL, and peroxidation of the unsaturated fatty acids was observed. SOD and 1O2 scavengers also inhibited the peroxidation of arachidonate, indicating that 1O2 generated in the xanthine oxidase system participates in the peroxidation reaction.