Characterization of epidermal growth factor receptor in normal and neoplastic human endometrium
Open Access
- 1 November 1990
- Vol. 66 (9) , 1967-1974
- https://doi.org/10.1002/1097-0142(19901101)66:9<1967::aid-cncr2820660920>3.0.co;2-1
Abstract
Growth factors, including epidermal growth factor (EGF), have been implicated in the growth of several types of cancer. This study compares EGF receptors in normal and neoplastic endometrium. Membrane fractions were isolated from surgical specimens. Radioreceptor assays demonstrated the presence of receptors with a dissociation constant of 0.64 nmol/1 in normal endometrium. Affinity crosslinking revealed receptor molecular weight of 150 to 170 kiloDaltons (KD). A survey of samples (n = 37) revealed progressive decrease of EGF receptors in cancers of increasing grade: Grade 1–2 adenocarcinoma decreased 34% from control (n = 6, P < 0.01), whereas Grade 3 adenocarcinoma decreased 90% (n = 7, P P < 0.01). The dissociation constant and molecular weight of the receptor in neoplastic endometrium did not differ significantly from normal. The inverse relationship with grade suggests receptor alteration or down regulation by hormones and/or growth factors.This publication has 37 references indexed in Scilit:
- Growth factors modulate junctional cell-to-cell communicationThe Journal of Membrane Biology, 1988
- Different epidermal growth factor growth responses and receptor levels in human colon carcinoma cell linesCancer Letters, 1988
- Human squamous cell lung cancers express increased epidermal growth factor receptors.Journal of Clinical Investigation, 1984
- Growth factors: Mechanism of action and relation to oncogenesCell, 1984
- Amplification and Enhanced Expression of the Epidermal Growth Factor Receptor Gene in A431 Human Carcinoma CellsScience, 1984
- EGF receptor binding studies in endometrial cell cultureLife Sciences, 1984
- Close similarity of epidermal growth factor receptor and v-erb-B oncogene protein sequencesNature, 1984
- Transformation by murine and feline sarcoma viruses specifically blocks binding of epidermal growth factor to cellsNature, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- THE ATTRACTIONS OF PROTEINS FOR SMALL MOLECULES AND IONSAnnals of the New York Academy of Sciences, 1949