PROBLEM OF REGULATION OF PROTEASE SYNTHESIS IN BACILLUS-SUBTILIS A-50

  • 1 January 1975
    • journal article
    • research article
    • Vol. 11  (7) , 135-144
Abstract
The effect of glucose and amino acids on alkaline protease synthesis of prototrophs and auxotrophs of B. subtilis A-50 was studied. General and specific regulation mechanisms for sporulation and protease synthesis were noted during early sporulation stages. Most auxotrophic mutations pleiotropically decreased the level of protease synthesis and the efficiency of sporulation. Different levels of sporulation and protease formation induced by glucose, amino acids and casein amino acids were also observed. The increase in glucose concentration in the medium from 0.5 to 5% led to an increase of protease synthesis and a decrease of sporulation efficiency. The suppression of alkaline protease formation in B. subtilis occured when individual amino acid concentrations in the mixture were on the order of 1.5 mg/ml in the presence of 1.5% glucose. The strongest inhibitory effect was found in 2nd[Val, Leu, Gly, Ala], 3rd[Ile, Ser, Pro, Thr] and 4th[Asn, Gln, Asp, Glu] amino acid groups in the presence of 5% glucose, whereas amino acids of the 1st group [Arg, His, Met, Lys] stimulated protease synthesis in the presence of 2% glucose. The amino acid stimulatory effect of the 1st group was due to histidine and arginine.

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