Ser1901 of α1C subunit is required for the PKA‐mediated enhancement of L‐type Ca2+ channel currents but not for the negative shift of activation
Open Access
- 26 January 2001
- journal article
- Published by Wiley in FEBS Letters
- Vol. 489 (1) , 87-91
- https://doi.org/10.1016/s0014-5793(01)02079-8
Abstract
Cardiac L‐type Ca2+ channel is facilitated by protein kinase A (PKA)‐mediated phosphorylation. Here, we investigated the role of Ser1901, a putative phosphorylation site in the carboxy‐terminal of rat brain type‐II α1C subunit (rbCII), in the PKA‐mediated regulation. Forskolin (3 μM) enhanced Ca2+ channel currents (I Ca) and shifted the activation curve to negative voltages, which were abolished by protein kinase inhibitor. Replacement of Ser1901 of rbCII by Ala abolished the enhancement of I Ca by forskolin but not the shift of the activation curve. These results indicate that Ser1901 is required for the PKA‐mediated enhancement of I Ca, and that the voltage‐dependence of the activation of I Ca appears to be modulated via another PKA phosphorylation site.Keywords
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