Selection and In Vivo Properties of Lectin-Attachment Variants of Malignant Murine Lymphosarcoma Cell Lines2

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Abstract
Variants of malignant RAW117 lymphosarcoma cell lines were selected by sequential nonadherence to polystyrene-immobilized lectins. Parental murine lymphosarcoma line RAW-117-P grew in suspension and was rapidly bound by immobilized concanavalin A (Con A), Rlclnus communis agglutinin I (RCA1), peanut agglutinin (PNA), or wheat germ agglutinin (WGA). After attachment of most cells to lectin-substituted petri dishes, the unattached cells (200) liver colonies. RAW117-P cells selected by nonadherence on immobilized WGA, PNA, or RCAI showed no increase in liver tumor formation compared to the cells of the parental line. In contrast, RAW117-H10, a variant line selected ten times in vivo for liver colonization, formed more than 200 liver tumor colonies, and selection eight times on immobilized WGA yielded a line (RAW117-H10 WGAa8) that formed a median of only 1 (range, 0–4) tumor colony. Variants of the RAW117-H10 line selected on immobilized RCAI, Con A, or PNA still formed more than 200 liver tumor colonies.125l-labeled lectin-binding studies indicated decreased lectin-binding sites on the in vivo and some of the immobilized lectin-selected lines. Autoradiographs of125l-labeled Con A-stained sodium dodecyl sulfate polyacrylamide gels showed a decrease in the major Con A-staining band (mol wt, ≈70,000) in the in vivo and immobilized Con A-selected lines. These studies indicated that alterations in cell surface lectin receptors could modify in vivo malignant behavior.

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