Solution conformation of leiurotoxin I (scyllatoxin) by 1H nuclear magnetic resonance
- 29 January 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 260 (2) , 249-253
- https://doi.org/10.1016/0014-5793(90)80115-y
Abstract
A proton NMR study at 500 MHz of leiurotoxin I in water is presented. Nearly complete sequence‐specific assignments of the individual backbone and side‐chain proton resonances were achieved using through‐bond and through‐space connectivities obtained from standard two‐dimensional NMR techniques. The secondary structure of this toxin is inferred from a combination of short‐range nuclear Overhauser enhancements, scalar couplings and proton/deuteron exchange rates. Three disulflde bridges locate the N‐terminal part (that is α‐helical from residue 6 to 16) on one side of a C‐terminal two stranded antiparallel β sheet (from Leu18 to Val29). The latter features a tight turn at Gly23‐Asp24.Keywords
This publication has 20 references indexed in Scilit:
- Spin-echo water suppression for the generation of pure-phase two-dimensional NMR spectraJournal of Magnetic Resonance (1969), 1987
- Identification of two toxins from scorpion (Leiurus quinquestriatus) venom which block distinct classes of calcium‐activated potassium channelFEBS Letters, 1986
- Leiurus quinquestriatus venom inhibits different kinds of Ca2+-dependent K+ channelsBiochimica et Biophysica Acta (BBA) - Biomembranes, 1986
- MLEV-17-based two-dimensional homonuclear magnetization transfer spectroscopyJournal of Magnetic Resonance (1969), 1985
- Practical aspects of two-dimensional transverse NOE spectroscopyJournal of Magnetic Resonance (1969), 1985
- Improved spectral resolution in COSY 1H NMR spectra of proteins via double quantum filteringBiochemical and Biophysical Research Communications, 1983
- Coherence transfer by isotropic mixing: Application to proton correlation spectroscopyJournal of Magnetic Resonance (1969), 1983
- Application of phase sensitive two-dimensional correlated spectroscopy (COSY) for measurements of 1H-1H spin-spin coupling constants in proteinsBiochemical and Biophysical Research Communications, 1983
- A two-dimensional nuclear Overhauser enhancement (2D NOE) experiment for the elucidation of complete proton-proton cross-relaxation networks in biological macromoleculesBiochemical and Biophysical Research Communications, 1980
- Structure-function relations and site of action of apamin, a neurotoxic polypeptide of bee venom with an action on the central nervous systemBiochemistry, 1975