Characterization of a Monoclonal Antibody Directed Against the Phosphotyrosine Kinase p56lck, in Human T Cells
- 1 April 1991
- journal article
- Published by Wiley in Scandinavian Journal of Immunology
- Vol. 33 (4) , 375-380
- https://doi.org/10.1111/j.1365-3083.1991.tb01784.x
Abstract
A monoclonal antibody (anti-p56lck) was generated against a fusion protein containing the residues 145-509 of the human p56lck, a lymphocyte-specific membrane-associated protein tyrosine kinase. The involvement of this enzyme in T-cell transmembrane signalling seems to be an early and crucial event during T-cell receptor-mediated activation. We have produced a monoclonal antibody which recognizes p56lck in free form and when associated with CD4. It functions in western blot analysis and is capable of selectively blocking auto-phosphorylation of this kinase. This monoclonal antibody should be useful for investigating the role of p56lck in T-cell activation.Keywords
This publication has 26 references indexed in Scilit:
- A New Surface Antigen on Human Lymphocytes Defined by the Murine Monoclonal Antibody IVF7Scandinavian Journal of Immunology, 1990
- T Cell Antigen Receptor-Mediated Activation of Phospholipase C Requires Tyrosine PhosphorylationScience, 1990
- Do CD4 and CD8 control T-cell activation via a specific tyrosine protein kinase?Immunology Today, 1989
- The CD4 and CD8 T cell surface antigens are associated with the internal membrane tyrosine-protein kinase p56lckCell, 1988
- The amino terminal region of the p56 lck from LSTRA exerts negative modulation on the tyrosine kinase activityBiochemical and Biophysical Research Communications, 1987
- Identification of the human T-lymphocyte protein-tyrosine kinase by peptide-specific antibodiesBiochemical and Biophysical Research Communications, 1986
- Human T lymphocytes express a protein-tyrosine kinase homologous to p56LSTRABiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1986
- A human T cell‐specific cDNA clone (YT16) encodes a protein with extensive homology to a family of protein‐tyrosine kinasesEuropean Journal of Immunology, 1986
- A lymphocyte-specific protein-tyrosine kinase gene is rearranged and overexpressed in the murine T cell lymphoma LSTRACell, 1985
- Protein iodination with solid state lactoperoxidaseBiochemistry, 1974