Enzyme-Linked Immunosorbent Assay for Apolipoprotein C-I

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Abstract
A non-competitive sandwich enzyme-linked immunosorbent assay for apolipoprotein C-I was developed. Sheep antibody to this apolipoprotein C-I, purified by affinity chromatography, was used for coating the wells of a microtiter plate and as a conjugate with alkaline phosphatase. The linear range of the assay was from 80 ng to 15 ng. It was sensitive down to 5 ng. The intra-assay variation coefficient was 2.8% and the inter-assay variation coefficient 5.3%. The mean concentration of apolipoprotein C-I was 61 .+-. 20 mg/l in healthy normal males, and 65 .+-. 19 mg/l in females. Apolipoprotein C-I levels were positively correlated with the total cholesterol concentration in both sexes (p < 0.002). A significant correlation with triacylglycerol was only observed in males (p < 0.05). A significant increase of apolipoprotein C-I was observed in type V hyperlipoproteinaemia, and in the only studied case of type III.