The two main saponins of commercially available extract of Primula sp. were isolated by HPLC. Their structures were elucidated by spectroscopic methods as already known primulasaponin (1) and the new 3-O-{[α-rhamnopyranosyl-(1→2)-β-galactopyranosyl-(1→3)-[β-xylopyranosyl-(1→4)-β-glucopyranosyl-(1→2)]-β-glucuronopyranosyl]]-protoprimulagenin A (2).