13C NMR Studies of Histidine Fermentation with aCorynebacterium glutamicumMutant

1 February 1986

journal article
research article
Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry

Vol. 50 (2) , 307-310
https://doi.org/10.1080/00021369.1986.10867392

Abstract

¹³C NMR spectroscopy was applied to studies on histidine biosynthesis in Corynebacterium glutamicum N-730, a histidine producing mutant. When it was cultured in a medium containing [1-¹³C]glucose as the carbon source, the 13C isotopomer was abundantly incorporated into C-6 (28 atom%) and C-l (21%), and to a lesser extent into C-5 (7%) of the histidine accumulated. The high incorporation into C-6 was explained as being due to that most of the one-carbon unit for histidine biosynthesis is derived from serine. On the bases of this view and the ¹³C population value at C-6, the contributions of the Embden-Meyerhof and hexose monophosphate pathways to the histidine production were calculated to be 56 and 44%, respectively. The significance of the label incorporation into C-l and C-5 was discussed.

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