Production of α-keto acids Part I. Immobilized cells ofTrigonopsis variabilis containing D-amino acid oxidase
- 1 December 1981
- journal article
- Published by Springer Nature in Applied Biochemistry and Biotechnology
- Vol. 6 (4) , 293-307
- https://doi.org/10.1007/bf02798280
Abstract
Whole cells ofTrigonopsis variabilis were immobilized by entrapment in Ca2+-alginate and used for the production of α-keto acids from the corresponding D-amino acids. The D-amino acid oxidase within the immobilized cells has a broad substrate specificity. Hydrogen peroxide formed in the enzymatic reaction was efficiently hydrolyzed by manganese oxide co-immobilized with the cells. The amino acid oxidase activity was assayed with a new method based on reversed-phase HPLC. Oxygen requirements, bead size, concentration of cells in the beads, flow rate, and other factors were investigated in a “ trickle-bed ” reactor.Keywords
This publication has 12 references indexed in Scilit:
- Enzyme thermistor determination of glucose in serum using immobilized glucose oxidaseClinica Chimica Acta; International Journal of Clinical Chemistry, 1977
- The immobilization of microbial cells, subcellular organelles, and enzymes in calcium alginate gelsBiotechnology & Bioengineering, 1977
- Continuous production ofL-malic acid by immobilizedBrevibacterium ammoniagenes cellsApplied Microbiology and Biotechnology, 1976
- Purification of d-amino acid oxidase from Trigonopsis variabilisAnalytical Biochemistry, 1976
- [32] The glucose oxidase—catalase systemPublished by Elsevier ,1976
- [29] Kinetic behavior of immobilized enzyme systemsPublished by Elsevier ,1976
- Continuous production of urocanic acid by immobilized Achromobacter liquidum cellsBiotechnology & Bioengineering, 1974
- Immobilized D-Amino Acid Oxidase Preparation, Some Enzymatic Properties, and Potential UsesAgricultural and Biological Chemistry, 1974
- Factors Affecting the Nitrogen Balance in Chronic Uremic Patients Receiving Essential Amino Acids Intravenously or by MouthAnnals of Nutrition and Metabolism, 1972
- Effect of the Microenvironment on the Mode of Action of Immobilized EnzymesPublished by Wiley ,1971