STUDIES ON PEPTIDES .111. SYNTHESIS OF CHICKEN GASTRIN-RELEASING PEPTIDE

  • 1 January 1982
    • journal article
    • research article
    • Vol. 20  (3) , 276-288
Abstract
The heptacosapeptide amide corresponding to the entire amino acid sequence of chicken gastrin-releasing peptide (cGRP) was synthesized similarly to the synthesis of porcine GRP by assembling 6 peptide fragments followed by deprotection with 1 M trifluoromethanesulfonic acid-thioanisole in TFA [trifluoroacetic acid]. A new carboxyl-activating reagent, thiazolidine-2-thione, was preferentially adopted for preparation of necessary fragments. The synthetic cGRP, purified by ion-exchange chromatography, followed by partition chromatography, was active as the synthetic procine GRP, when plasma immunoreactive gastrin level was examined in rats. No obvious difference was observed when synthetic and natural cGRP preparations were compared by HPLC [high performance liquid chromatography], immunochemical property and biological activity in dogs.