Preservation of Nucleic Acids for Polymerase Chain Reaction After Prolonged Storage at Room Temperature

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Abstract
A guanidinium isothiocyanate (GITC) lysis solution was evaluated for its efficacy in preserving nucleic acids for subsequent analysis after prolonged storage at room temperature. Aliquots of thyroid crude cell lysates were stored at 22oC for 8 weeks in the GITC solution. Crude cell lysates stored for 2 weeks in the GITC solution consistently provided adequate RNA and DNA for reverse transcriptase-polymerase chain reaction (RT-PCR) and PCR, respectively, of [beta]2-microglobulin, thyroid stimulating hormone receptor (TSHR), and thyroglobulin. Interestingly, the thyroglobulin and TSHR messages from thyroid fine needle aspirate samples were detected by RT-PCR only when stored at room temperature for less than 1 week, whereas the RT-PCR product for [beta]2-microglobulin was detectable in 95|X% of the samples at 3 months. In addition, thyroglobulin DNA was amplified by PCR in nearly all samples stored at 22oC for 2 months. These data suggest that GITC solutions can be used to preserve nucleic acids in most circumstances until trans-ported to laboratories equipped and staffed with appropriate resources (C) 1998 Lippincott Williams & Wilkins, Inc.