Antiviral Effect of TNF-α and IFN-γ Secreted from a CD8+Influenza Virus-Specific CTL Clone

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Abstract
We observed that an influenza-specific cytotoxic T lymphocyte (CTL) clone (B7B7) stimulated with peptide-antigen could produce TNF-α and IFN-γ simultaneously. The culture supernatant containing both TNF-α and IFN-γ of antigen-stimulated CTL clone B7B7 significantly enhanced the lysis of influenza A/PR/8 virus-infected L-M2d6 cells or Meth A cells. Enhanced lysis of influenza virus-infected cells by the supernatants was inhibited by pretreatment of the supernatant with antimurine TNF-α antibody and antimurine IFN-γ antibody. In addition to a single CTL clone, we observed that bulk-cultured CTLs were able to produce TNF and IFN when incubated with target cells. These results suggest that the protective mechanism mediated by TNF-α and IFN-γ secreted from CTL may be possible in the course of an influenza infection.