Efficient Mechanical Inoculation of Turnip Yellow Mosaic Virus Using Small Volumes of Inoculum

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Abstract
When purified turnip yellow mosaic virus was inoculated mechanically on to Chinese cabbage leaves, using known numbers of virus particles in 0.1-1.0 .mu.l volumes of inoculum, as few as 10-30 particles were required to produce a single local lesion. A simple procedure is described for obtaining local lesions from the virus in single infected protoplasts.

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