Production of formyl‐CoA during peroxisomal α‐oxidation of 3‐methyl‐branched fatty acids
- 28 April 1997
- journal article
- Published by Wiley in FEBS Letters
- Vol. 407 (2) , 197-200
- https://doi.org/10.1016/s0014-5793(97)00343-8
Abstract
α-Oxidation of 3-methyl-substituted fatty acids was studied in purified rat liver peroxisomes. The experiments revealed that formyl-CoA is formed during the α-oxidation process. The amount of formyl-CoA found constituted 2–5% of the amount of formate formed. Under the conditions used, no activation of exogenously added formate occurred in purified peroxisomes, whereas 95.5% of added synthetic formyl-CoA was converted to formate. These data indicate that during α-oxidation first formyl-CoA is formed, which is then hydrolysed to formate.Keywords
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