Some Properties of Human Erythrocyte Pyrimidine 5'-Nucleotidase
- 1 July 1981
- journal article
- research article
- Published by S. Karger AG in Enzyme
- Vol. 26 (2) , 64-73
- https://doi.org/10.1159/000459152
Abstract
In haemolysates human erythrocyte pyrimidine 5'-nucleotidase had a single optimum at pH 7.2 with CMP and at 6.75 with UMP as substrate. The purified enzyme showed two pH optima at pH 6.25 and 7.2 with UMP as substrate. The enzyme was inhibited by both its products - inorganic phosphate and pyrimidine nucleoside. The inhibition by inorganic phosphate appeared to be non-competitive with K(i) = 1.5 mM. Contrary to previous reports adenosine and inosine did not inhibit the enzyme.Keywords
This publication has 2 references indexed in Scilit:
- Purification and properties of human erythrocyte pyrimidine 5'-nucleotidase.Proceedings of the National Academy of Sciences, 1977
- SIMPLE RAPID RADIOMETRIC ASSAY FOR PYRIMIDINE-5'-NUCLEOTIDASE1977