Enzymatic hydrolysis of triglycerides byRhizopus delemarimmobilized on biomass support particles
- 1 January 1992
- journal article
- research article
- Published by Taylor & Francis in Food Biotechnology
- Vol. 6 (1) , 1-18
- https://doi.org/10.1080/08905439209549818
Abstract
The best medium composition for production of intracellular Iipase by Rhizopus delemar contained oleic acid and no glucose. The technique of cell immobilization using polyurethane foam biomass support particles was studied as a means of further enhancing intracellular lipase activity. Fungal mycelia were found to strongly adhere to the polyurethane foam matrix during growth to form immobilized cell biocataiysts, which, after cell membrane permeabiiization and drying, exhibited a Iipase activity 3.3 times greater than that of cells grown in a freely suspended state. Upase within the Immobilized cells showed properties similar to purified extracellular Rhizopus delemar lipase but was more thermostable than the free enzyme. The biocatalyst was used for triglyceride hydrolysis in a continuous stirred‐tank reactor. In this format it was found that the degree of hydrolysis of olive oil and the flavor profile of lipolyzed milk fat could be easily controlled.Keywords
This publication has 27 references indexed in Scilit:
- Nutritional factors affecting lipase production byRhizopus delemar CDBB H313Biotechnology Letters, 1990
- Kinetic studies on the interesterification of oils and fats using dried cells of fungusJournal of Fermentation Technology, 1988
- Methanogenesis by methanogen species immobilised on reticulated foam biomass support particles. Inhibition of methane production at high substrate concentrationsBiotechnology Letters, 1987
- The production of cellulase in a spouted bed fermentor using cells immobilized in biomass support particlesBiotechnology & Bioengineering, 1986
- Hydrolysis of triglyceride by solid phase lipolytic enzymes ofRhizopus arrhizus in continuous reactor systemsBiotechnology & Bioengineering, 1981
- ROLE OF SOME FATTY ACIDS ON THE GROWTH AND LIPASE PRODUCTION BY Streptococcus faecalisJournal of Food Science, 1979
- Synthesis of glycerides and esters by fungal cell-bound enzymes in continuous reactor systemsBiotechnology Letters, 1979
- Biological particles of given size, shape, and density for use in biological reactorsBiotechnology & Bioengineering, 1979
- Hydrolysis of particulate tributyrin in a fluidized lipase reactorBiotechnology & Bioengineering, 1975
- STUDIES ON LIPASEThe Journal of General and Applied Microbiology, 1964