Characterization of the type IV restriction modification system BspLU11III from Bacillus sp. LU11
- 15 November 2001
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 29 (22) , 4691-4698
- https://doi.org/10.1093/nar/29.22.4691
Abstract
We report the characterization and cloning of the genes for an unusual type IV restriction-modification system, BspLU11III, from Bacillus sp. LU11. The system consists of two methyltransferases and one endonuclease, which also possesses methyltransferase activity. The three genes of the restriction-modification system, bsplu11IIIMa, bsplu11IIIMb and bsplu11IIIR, are closely linked and tandemly arranged. The corresponding enzymes recognize the dsDNA sequence 5'-GGGAC-3'/5'-GTCCC-3', with M.BspLU11IIIa modifying the A (underlined) of one strand and M.BspLU11IIIb the inner C (underlined) of the other strand. R.BspLU11III has both endonuclease and adenine-specific methyltransferase activities and is able to protect the DNA against cleavage by itself. In contrast to all type IV restriction-modification systems described so far, which have only one adenine-specific methyltransferase, BspLU11III is the first type IV restriction-modification system that includes two methyltransferases, one of them being cytosine specific.Keywords
This publication has 35 references indexed in Scilit:
- Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genesPublished by Elsevier ,2004
- High efficiency transformation of Escherichia coli with plasmidsPublished by Elsevier ,2003
- The Hae IV restriction modification system of Haemophilus aegyptius is encoded by a single polypeptide 1 1Edited by M. GottesmanJournal of Molecular Biology, 1999
- Two intertwined methylation activities of the MmeI restriction–modification class-IIS system from Methylophilus methylotrophusGene, 1998
- Primary structure and strand specificity of BstF5I-1 DNA methyltransferase which recognizes 5′-GGATG-3′Gene, 1997
- Structure-guided Analysis Reveals Nine Sequence Motifs Conserved among DNA Amino-methyl-transferases, and Suggests a Catalytic Mechanism for these EnzymesJournal of Molecular Biology, 1995
- Basic local alignment search toolJournal of Molecular Biology, 1990
- Nucleotide sequence of the gene coding for the BanIII DNA methyltransferase in Bacillus aneurinolyticus.Agricultural and Biological Chemistry, 1990
- Cytosine-specific type II DNA methyltransferasesJournal of Molecular Biology, 1989
- Sequence motifs specific for cytosine methyltransferasesGene, 1988