LACK OF SUBCLASS SELECTION DURING DENSITY PURIFICATION OF HUMAN LYMPHOCYTES

  • 1 January 1977
    • journal article
    • research article
    • Vol. 28  (2) , 341-346
Abstract
The percentage of acid .alpha.-naphthyl acetate esterase (ANAE) marker-carrying lymphocytes (T [thymus-derived] lymphocytes) was determined directly from the blood, after density purification of lymphocytes over Ficoll-Isopaque and removal of monocytes of Fe powder plus magnet. The effect of the Ficoll-Isopaque centrifugation was insignificant; neither did the Fe powder plus magnet procedure increase the relative proportion of the T cells in these preparations. In optimal conditions there is no subclass-specific enrichment of T or B [bone marrow-derived] lymphocytes during the purification procedure.