HPTLC Separation and Densitometric Determination of Some Metallic Dithizonates at Subnanogram Level: Applications to Real Samples

Publisher Website
Google Scholar
Abstract
Some metallic ions have been separated as dithizonates and determined in situ by densitometric automatic scanning of the plate in quantities down to 0.5 ng. High efficiency plates have been employed whose theoretical plate height relative to the chosen conditions was found to be about 20 μ. Rf values of Co, Pb, Cu, Ni, Zn, Hg are 0.23, 0.38, 0.40, 0.46, 0.53, 0.65 respectively. Conditioning by H2SO4 leads to improvement in the separation and conditioning by NH3 separates Hg from all other dithizonates. Some examples of real sample analysis (Zn, Co, Ni in soaps, Vitamin B12, Hg in human urine) are reported.