PRODUCTION OF PERITHECIA OF VENTURIA INAEQUALIS (CKE.) WINT. ON STERILE APPLE LEAF DISCS

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Abstract
Perithecia of Venturia inaequalis were produced in vitro on apple leaf discs which had been sterilized in propylene oxide and then placed on water agar, potato-dextrose agar, or potato-dextrose agar supplemented with apple leaf decoction. The discs were inoculated with a drop of a conidial suspension of two sexually compatible strains of V. inaequalis, incubated at room temperature for 2 weeks, and then grown at 8 °C for 4 to 5 months.Perithecia were also produced on sterile leaf discs placed on perlite, vermiculite, sand, filter paper, or glass rings in screw-cap vials with distilled water added to supply moisture. Using perlite, perithecia were developed on discs of green and fallen apple leaves collected from the orchard at intervals during the summer and fall and on leaves at different stages of development from trees growing in the greenhouse. Few perithecia developed on newly unfolded leaves from the latter unless the leaves were leached before inoculation. Perithecia developed on sterile discs from green apple leaves of varieties with varying degrees of susceptibility to V. inaequalis and on green leaf discs of the pear variety Kieffer. Perithecia production was most abundant at 4 °C and no perithecia were produced at or above 15 °C.

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