Antisera of designed specificity for subunits of guanine nucleotide-binding regulatory proteins.
- 1 January 1986
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 83 (2) , 265-269
- https://doi.org/10.1073/pnas.83.2.265
Abstract
Antisera were raised against purified subunits of regulatory GTP-binding proteins (G proteins) and against synthetic peptides that correspond to defined regions of G proteins. Peptide antisera were generated that recognized all .alpha. or all .beta. subunits from Gs, Gi, Go, and transducin; others recognized only Gs.alpha. or Go.alpha.. Such cross-reaction or complete specificity for a given .alpha. subunit was not obtained when purified subunits were injected. Peptide antisera were used to identify G protein subunits in selected tissue membrane preparations by immunoblots.Keywords
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