LOCALIZATION OF THE NA+/K+-ATPASE AND OF AN AMILORIDE SENSITIVE NA+ UPTAKE ON THYROID EPITHELIAL-CELLS

  • 1 January 1985
    • journal article
    • research article
    • Vol. 38  (1) , 134-141
Abstract
The Na+/K+-ATPase was localized using purified specific antibodies on the basolateral membranes of rat thyroid epithelial cells and of cultured porcine thyroid cells, by immunofluorescence and immunoelectron microscopy. No staining was observed on the apical membranes. When cultured cells formed monolayers, with their apical pole in contact with the culture medium, 22Na+ uptake was inhibited by amiloride. Inhibition was dependent upon extracellular Na+ concentration, half maximal inhibition was obtained with 0.7 .mu.M amiloride in the presence of 5 mM Na+. Ouabain was ineffective on Na+ uptake into intact monolayers. A brief treatment of the monolayers with ethyleneglycol-bis(.beta.-aminoethyl ether)-N,N,N'',N''-tetraacetic acid (EGTA) opened the tight junctions and allowed the access of ouabin to the basal pole of the cells. In this condition ouabain increased Na+ uptake. When cells were recognized into follicle-like structures, with their basal pole in contact with the culture medium, Na+ uptake was not modified by amiloride but was increased by ouabain. We conclude that in thyroid cells, the Na+/K+-ATPase is present on the basolateral domain of the plasma membrane whereas an amiloride sensitive sodium uptake occurs at the apical surface.