Characterization of the Metallothioneins Induced in HeLa Cells by Dexamethasone and Zinc
Open Access
- 1 June 1980
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 107 (2) , 395-401
- https://doi.org/10.1111/j.1432-1033.1980.tb06042.x
Abstract
The metallothioneins induced in HeLa cells by dexamethasone and Zn2+ were labeled with [3H]cysteine and [35S]cysteine respectively. These labeled metallothioneins were compared to one another and to metallothioneins from human liver by (a) gel permeation chromatography on Sephadex G-75 columns, (b) ion-exchange chromatography on DEAE-Sephadex A-25 columns, (c) electrophoresis on non-denaturing polyacrylamide gels, and (d) electrophoresis of reduced and alkylated proteins on dodecylsulfate/polyacrylamide gels. The dexamethasone-induced metallothioneins of HeLa cells, the Zn2+-induced metallothioneins of HeLa cells, and the metallothioneins of human liver were indistinguishable by these four criteria.This publication has 32 references indexed in Scilit:
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