ENZYME-LINKED IMMUNOASSAY FOR PLACENTAL-LACTOGEN IN HUMAN-SERUM

  • 1 January 1980
    • journal article
    • research article
    • Vol. 26  (6) , 745-749
Abstract
An enzyme-linked immunoassay for measuring human placental lactogen (HPL) in serum is described. After suitable dilution, sera are pipetted into the wells of polyvinyl microtiter plates previously coated with anti-HPL. After incubation, the sera are decanted and replaced by a conjugate of horseradish peroxidase and anti-HPL. An amount of enzyme proportional to the concentration of HPL in the serum is bound to the wells via an antibody-HPL-antibody bridge. Peroxidase activity is measured by oxidation of o-phenylenediamine, the resulting color being related to the concentration of HPL. Results correlate well with those by radioimmunoassay (correlation coefficient = 0.96). The between-assay coefficient of variation was 13%; no discernible effect of protein was observed. This 4 h assay can be used to monitor placental function in pregnancy.

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