Elimination OF Distortion and Poor Staining in Paraffin Sections OF Plant Tissues

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Abstract
Three fixing solns. causing least distortion and bright staining of plant tissues are named. Glycerin dehydration causes less distortion than a series of alcohol concs.; 95% alcohol removes some of the glycerin, sets the protoplasm and improves the staining. Absolute alcohol causes distortion and should be avoided. Pure chloroform, as a paraffin solvent, is followed by brighter staining but more distortion than are the butyl alcohols. A schedule resulting in minimum distortion is given. The results are shown in photomicrographs. Brightest staining follows the use of C. P. iron alum and hematoxylin. The use of a paper cup for very gradual change from one liquid to another and as a labor saver is descr.

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