Enhanced cell‐free transcription of the ribosomal protein L32 gene by the polyoma virus enhancer PEA3 DNA‐binding protein
- 1 July 1992
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 207 (1) , 195-200
- https://doi.org/10.1111/j.1432-1033.1992.tb17037.x
Abstract
The mouse-ribosomal-protein-L32-gene promoter contains a 12-bp sequence motif within the 5'-upstream region termed the beta element which shows significant similarity with the consensus sequence of the polyoma-virus-enhancer PEA3. A cloned PEA3 DNA-binding protein, expressed in Escherichia coli and purified, activates the expression of the ribosomal-protein-L32 gene in a cell-free system. Moreover, the PEA3 protein participates in the formation of the ribosomal-protein-L32-promoter-preinitiation-transcription complex. The preinitiation complex formed with PEA3 is resistant to competition by oligonucleotides containing the beta element. In addition anti-PEA3 serum interacts with a factor in mouse L1210 nuclear extract that binds to the beta element, causing a supershift in a mobility-shift assay. Our study demonstrates for the first time that the PEA3 protein can transactivate a cellular gene in a cell-free transcription system.Keywords
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