Activity-based protein profiling for the functional annotation of enzymes
- 27 September 2007
- journal article
- review article
- Published by Springer Nature in Nature Methods
- Vol. 4 (10) , 822-827
- https://doi.org/10.1038/nmeth1092
Abstract
Activity-based protein profiling (ABPP), the use of active site-directed chemical probes to monitor enzyme function in complex biological systems, is emerging as a powerful post-genomic technology. ABPP probes have been developed for several enzyme classes and have been used to inventory enzyme activities en masse for a range of (patho) physiological processes. By presenting specific examples, we show here that ABPP provides researchers with a distinctive set of chemical tools to embark on the assignment of functions to many of the uncharacterized enzymes that populate eukaryotic and prokaryotic proteomes.Keywords
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