Detection of styrene oxide—DNA adducts by 32P-postlabeling
- 1 August 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 9 (8) , 1401-1404
- https://doi.org/10.1093/carcin/9.8.1401
Abstract
In vitro reaction of DNA with styrene-7, 8-oxide (Styrene oxide) produced five adducts, as determined by 32 P-postlabeling. When styrene oxide was reacted in vitro with deoxyribonucleotieds, five adducts were observed from 2' -deoxyguanosine-3' -monophosphate, two from 2 -deoxythymidine-3-monophosphate none from 2' -deoxythymidine-3-monophosphate or 2' -deoxycytidine-3' -monophosphate. Chromatographic comparison of the adducts formed in DNA with those formed with the deoxyribonucleotides suggests that deoxyguanosine is the primary site of DNA modification. Treatment of 9L cells with 1 mM styrene oxide resulted in the formation of several DNA adducts as detected by the postlabeling procedure. Our results indicate that 32 P-postlabeling can be used to investigate DNA adducts formed by styrene oxide.This publication has 0 references indexed in Scilit: