Celery transformation by Agrobacterium tumefaciens: cytological and genetic analysis of transgenic plants
- 1 March 1988
- journal article
- research article
- Published by Springer Nature in Plant Cell Reports
- Vol. 7 (2) , 100-103
- https://doi.org/10.1007/bf00270114
Abstract
Transgenic celery plants were obtained following co-cultivation of petiole explants with Agrobacterlum tumefaciens containing pMON200, a cointegrate vector carrying genes for kanamycin resistance and nopaline synthase. Transformants were selected by ability of callus to grow in the presence of 50mg/l kanamycin. Transformation was confirmed either by the presence of nopaline or by Southern blots. Cytological analysis of 14 transformed plants revealed chromosomal aberrations, both in structure and number. Only 20% of the regenerated plants had the normal karyotype. Kanamycin resistance behaved as a monogenic, dominant trait, segregating in a 3:1 ratio in three families derived from plants with normal karyotypes.Keywords
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