USE OF THE ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) FOR THE DETECTION AND QUANTIFICATION OF SPECIFIC ANTIBODY FROM CELL-CULTURES

  • 1 January 1979
    • journal article
    • research article
    • Vol. 37  (1) , 45-52
Abstract
The solid phase ELISA qas used to quantify anti-keyhole limpet hemocyanin (anti-KLH) antibody in the serum of KLH-immune C57B1/6 mice. When spleen cells from immune mice were cultured overnight in ELISA microtiter wells where KLH was, easily quantifiable amounts of anti-KLH antibody were synthesized and detected. Spleen cells from KLH-primed mice, when cultured in vitro in the presence of KLH, transferred to KLH-labeled ELISA plates, and cultured overnight, produced detectable levels of antibody. Levels of antibody were detectable after 4 and 5 days of in vitro stimulation. A comparison was made between detectable numbers of plaque forming cells to sheep red blood cells (SRBC) in SRBC primed CBA mice and levels of antibody detected by the ELISA procedure. The sensitivities of the 2 tests were comparable. The applications of this technique to the study of in vitro antibody synthesis using soluble antigens were discussed.