Complete assignment of the 1H NMR spectrum and secondary structure of the DNA binding domain of GAL4
- 10 December 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 276 (1-2) , 49-53
- https://doi.org/10.1016/0014-5793(90)80504-c
Abstract
Complete 1H NMR resonance assignments are presented for the cysteine rich region of the DNA binding domain of the yeast transcriptional activator GAL4. The protein contains short helical regions between Asp‐12 and Leu‐19 and between Lys‐30 and Trp‐36. It is clearly distinct from the C2H2 class of zinc finger protein typified by the Xenopus laevis transcription factor (TF)IIIA. We also find that the first SP(X)(X) sequence, a recently proposed DNA binding motif (residues 41 to 44), appears to be tightly packed against the metal binding domain.Keywords
This publication has 26 references indexed in Scilit:
- SPXX, a frequent sequence motif in gene regulatory proteinsJournal of Molecular Biology, 1989
- Baseline distortion in real-fourier-transform NMR spectraJournal of Magnetic Resonance (1969), 1988
- Iterative schemes for bilinear operators; application to spin decouplingJournal of Magnetic Resonance (1969), 1988
- Improved techniques for homonuclear rotating-frame and isotropic mixing experimentsJournal of Magnetic Resonance (1969), 1987
- Differentiation of direct and remote connectivities in phase-sensitive double-quantum spectra of proteins by variation of the multiple-quantum excitation periodJournal of Magnetic Resonance (1969), 1986
- Analysis of 1H NMR spectra of proteins using multiple-quantum coherenceJournal of Magnetic Resonance (1969), 1986
- Nuclear magnetic resonance identification of “half-turn” and 310-helix secondary structure in rabbit liver metallothionein-2Journal of Molecular Biology, 1986
- Application of phase sensitive two-dimensional correlated spectroscopy (COSY) for measurements of 1H-1H spin-spin coupling constants in proteinsBiochemical and Biophysical Research Communications, 1983
- Elimination of baseline artifacts in spectra and their integralsJournal of Magnetic Resonance (1969), 1983
- Multiple quantum spin-echo spectroscopyJournal of Magnetic Resonance (1969), 1980